[Eburu n'uche iji]

Dengue IgG/IgM Rapid Test Cassette bụ n'akụkụ eruba chromatog-raphic immunoassay maka nchọpụta qualitative nke ọgwụ mgbochi ọrịa (IgG na IgM) ka nje dengue n'ime ọbara dum/Serum/Plasma.Ọ na-enye aka na nchoputa nke ọrịa na nje Dengue.

[NKWUO]

Ahụ ọkụ Dengue bụ nnukwu ọrịa na-efe efe na-efe efe nke vector na-ebute site na nje dengue nke anwụnta na-ebute.Ọrịa nje virus dengue nwere ike ibute ọrịa recessive, ahụ ọkụ dengue, ahụ ọkụ hemorrhagic dengue, dengue hemorrhagic fever.Ihe ngosi a na-ahụkarị nke ahụ ọkụ dengue gụnyere mmalite mberede, oke ahụ ọkụ, isi ọwụwa, anụ ahụ siri ike, ọkpụkpụ na nkwonkwo mgbu, akpụkpọ anụ ahụ, ọbara ọgbụgba, mmụba nke ọnụ ọnụ lymph, mbelata ọnụ ọgụgụ ọbara ọcha, thrombocytopenia na ihe ndị ọzọ na ụfọdụ ndị ọrịa.Nke a na ọrịa ihu ọma bụ na okpomọkụ na subtropical ebe ewu ewu, n'ihi na ọrịa a na-ebunye site Aides anwụnta, mere ewu ewu nwere ụfọdụ seasonally, na-na kwa afọ ọtụtụ mgbe na May ~ November, ọnụ ọgụgụ kasị elu bụ na July ~ September.N'ebe ọhụrụ a na-efe efe, ndị mmadụ na-adịkarị mfe, mana ihe kpatara ya bụ ndị okenye, na mpaghara ebe a na-efe efe, ihe na-emekarị bụ ụmụaka.

[IWU]

Dengue IgG/IgM Rapid Test Cassette bụ qualitative membrane strip based immunoassay maka nchọpụta nke nje nje dengue (IgG na IgM) n'ime ọbara dum/Serum/Plasma mmadụ.Kaseti ule ahụ nwere: 1) pad conjugate na-acha agba burgundy nwere dengue recombinant envelopu antigens conjugated na Colloid gold (dengue conjugates), 2) a nitrocellulose membrane strip nwere ule abụọ (IgG na IgM ahịrị) na akara akara (C ahịrị). ).Ekpuchiri ahịrị IgM ahụ na mgbochi Mouse anti-Human IgM, akara IgG na-egbochi mgbochi mmadụ IgG.Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette, ihe nlere ahụ na-akwaga site na capillary edinam gafee cassette.IgM mgbochi dengue ma ọ bụrụ na ọ dị na ụdị ahụ ga-ejikọta na dengue conjugates.A na-ejide immunocomplex ahụ site na reagent mkpuchi na ahịrị IgM, na-etolite ahịrị IgM nwere agba burgundy, na-egosi nsonaazụ nyocha dengue IgM dị mma ma na-atụ aro ọrịa ọhụrụ.IgG mgbochi dengue, ọ bụrụ na ọ dị na ụdị ahụ, ga-ejikọta na dengue conjugates.A na-ejide immunocomplex ahụ site na reagent kpuchie ya na band IgG, na-eme ahịrị IgG nwere agba burgundy, na-egosi nsonaazụ nyocha dengue IgG dị mma ma na-atụ aro ọrịa na-adịbeghị anya ma ọ bụ megharịa.Enweghị ahịrị T ọ bụla (IgG na IgM) na-egosi nsonaazụ adịghị mma.Iji jee ozi dị ka njikwa usoro, eriri nwere agba ga-apụta mgbe niile na mpaghara ahịrị akara na-egosi na agbakwunyere olu kwesịrị ekwesị nke ihe nlere na akpụkpọ anụ ahụ emeela.

[Nchekwa na Nkwụsi ike]

Chekwaa dị ka akpachịkọtara n'ime obere akpa akara na okpomọkụ (4-30 ℃ ma ọ bụ 40-86 ℉).Ngwa ahụ kwụsiri ike n'ime ụbọchị njedebe nke ebipụtara na labeling.

Ozugbo meghere obere akpa ahụ, a ga-eji ule ahụ mee ihe n'ime otu awa.Ikpughe ogologo oge na gburugburu ebe dị ọkụ na iru mmiri ga-ebute mmebi ngwaahịa.

Ebipụtara LOT na ụbọchị njedebe na labeling.

[SPECIMAN]

Enwere ike iji ule a nwale nleba anya zuru oke nke ọbara/Serum/Plasma.

Iji nakọta ọbara zuru oke, serum ma ọ bụ plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.

Kewapụ serum ma ọ bụ plasma na ọbara ozugbo enwere ike iji zere hemolytic.Jiri naanị ihe nlere anya anaghị emebi emebi.

Chekwaa ihe nlele na 2-8℃ (36-46℉) ma ọ bụrụ na anwaleghị ya ozugbo.Na-echekwa ihe nlele na 2-8 ℃ ruo ụbọchị 7.Ekwesịrị ka oyi kpọnwụrụ ụdịdị ahụ na

-20℃ (-4℉) maka nchekwa ogologo.Akwụsịla ụdị ọbara dum.

Zenarị usoro ntu oyi na-agbaze ọtụtụ ugboro.Tupu anwale, weta ụdị oyi kpọnwụrụ n'ime ụlọ ahụ nwayọ wee gwakọta nwayọ.A ga-akọwapụta ihe nlere nwere obere ihe a na-ahụ anya site na centrifugation tupu nnwale.

Ejila ihe nlele na-egosi nnukwu onye lineman, nnukwu hemolytic ma ọ bụ turbidity iji zere ndabichi na nkọwa nsonaazụ.

[Usoro ule]

Kwe ka ngwaọrụ ule na ụdị nha nhata na okpomọkụ (15-30℃ ma ọ bụ 59-86℉) tupu anwale.

1. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya.Wepu cassette ule na obere akpa ahụ mechiri emechi wee jiri ya ozugbo enwere ike.

2. Tinye kaseti ule n'elu ala dị ọcha ma dị larịị.

3. Jide dropper kwụ ọtọ wee bufee 1 dobe nke ụdị (ihe dị ka 10μl) na ụdị nke ọma (S) nke cassette ule, wee tinye 2 tụlee nke ihe nchekwa (ihe dị ka 70μl) wee malite ngụ oge.Lee ihe atụ n'okpuru.

4. Chere ka ahịrị(s) nwere agba pụta.Gụọ nsonaazụ na nkeji iri na ise.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.