Ezubere iji

Dengue NS1 Ag-IgG/IgM Combo Test bụ ngwa ngwa chromatographic immunoassay maka nchọpụta qualitative nke ọgwụ nje (IgG na IgM) na dengue virus NS1 antigen ka nje dengue na Serum ma ọ bụ Plasma iji nyere aka na nchoputa nke nje virus Dengue.

[NKWUO]

Ahụ ọkụ Dengue bụ nnukwu ọrịa na-efe efe na-efe efe nke vector na-ebute site na nje dengue nke anwụnta na-ebute. Ọrịa nje virus dengue nwere ike ibute ọrịa recessive, ahụ ọkụ dengue, ahụ ọkụ hemorrhagic dengue, dengue hemorrhagic fever. Ihe ngosi a na-ahụkarị nke ahụ ọkụ dengue gụnyere mmalite mberede, oke ahụ ọkụ, isi ọwụwa, anụ ahụ siri ike, ọkpụkpụ na nkwonkwo mgbu, akpụkpọ anụ ahụ, ọbara ọgbụgba, mmụba nke ọnụ ọnụ lymph, mbelata ọnụ ọgụgụ ọbara ọcha, thrombocytopenia na ihe ndị ọzọ na ụfọdụ ndị ọrịa. Nke a na ọrịa ihu ọma bụ na okpomọkụ na subtropical ebe ewu ewu, n'ihi na ọrịa a na-ebunye site Aides anwụnta, mere ewu ewu nwere ụfọdụ seasonally, na-na kwa afọ ọtụtụ mgbe na May ~ November, ọnụ ọgụgụ kasị elu bụ na July ~ September. N'ebe ọhụrụ a na-efe efe na-efe efe, ndị mmadụ na-adịkarị mfe, mana ihe kpatara ya bụ ndị okenye, na mpaghara ebe a na-efe efe, ihe a na-emekarị bụ ụmụaka.

Ụkpụrụ

Dengue NS1 Ag-IgG/IgM Combo Test bụ qualitative membrane strip based immunoassay maka nchọpụta nke nje nje dengue (IgG na IgM) na dengue virus NS1 antigen na Serum ma ọ bụ Plasma.

Maka ule IgG/IgM: Ngwa nnwale ahụ nwere: 1) pad conjugate na-acha uhie uhie nke nwere dengue recombinant envelopu antigens conjugated na Colloid gold (dengue conjugates), 2) nitrocellulose akpụkpọ ahụ warara nke nwere ahịrị ule abụọ (T1 na T2 ahịrị) na akara akara (akara C). Ejiri mgbochi T1 kpuchie ya na mgbochi maka nchọpụta IgM mgbochi dengue, a na-ekpuchi akara T2 na mgbochi maka nchọpụta IgG anti-dengue. Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nlele nke cassette nlele ahụ, ihe atụ ahụ na-akwaga site na capillary edinam gafee cassette. IgG mgbochi dengue, ọ bụrụ na ọ dị na ụdị ahụ, ga-ejikọta na dengue conjugates. A na-ejide immunocomplex ahụ site na reagent kpuchiri ya na band T2, na-eme ahịrị T2 nwere agba burgundy, na-egosi nsonaazụ dengue IgG dị mma ma na-atụ aro ọrịa na-adịbeghị anya ma ọ bụ megharịa. IgM mgbochi dengue ma ọ bụrụ na ọ dị na ụdị ahụ ga-ejikọta na dengue conjugates. A na-ejide immunocomplex ahụ site na reagent mkpuchi na ahịrị T1, na-eme ahịrị T1 nwere agba burgundy, na-egosi nsonaazụ nyocha dengue IgM dị mma ma na-atụ aro ọrịa ọhụrụ. Enweghị ahịrị T ọ bụla (T1 na T2) na-egosi nsonaazụ na-adịghị mma.

Maka Nnwale NS1: N'ime usoro nnwale a, mgbochi Dengue NS1 anaghị emegharị ahụ na mpaghara akara ule nke cassette. Mgbe etinyechara ụdị ọbara zuru oke / serum / Plasma n'ime ihe nlele ahụ nke ọma, ọ na-emeghachi ihe mgbochi Dengue NS1 mgbochi mmadụ nke etinyere na pad ihe atụ. Ngwakọta a na-akwaga chromatographically n'ogologo nke mpempe ule wee na-emekọrịta ihe na mgbochi Dengue NS1 na-adịghị ahụkebe. Ọ bụrụ na ihe nlere ahụ nwere nje dengue NS1 antigen, ahịrị nwere agba ga-apụta na mpaghara ahịrị ule na-egosi nsonaazụ dị mma. Ọ bụrụ na ihe nlere ahụ enweghị nje dengue NS1 antigen, ahịrị nwere agba agaghị apụta na mpaghara a na-egosi nsonaazụ na-adịghị mma.

Iji rụọ ọrụ dịka njikwa usoro, ahịrị na-acha agba ga-apụta mgbe niile na mpaghara ahịrị akara na-egosi na agbakwunyere oke nlere nke ọma yana nhịahụ akpụkpọ ahụ emeela.

Nchekwa na kwụsie ike

Chekwaa dị ka akpachịkọtara n'akpa mkpuchi mkpuchi na ụlọ okpomọkụ ma ọ bụ friji (4-30 ℃ ma ọ bụ 40-86 ℉). Ngwaọrụ nnwale ahụ kwụsiri ike site na ụbọchị ngwụcha ebipụtara na obere akpa ahụ ejiri mechiri emechi.

Nnwale ahụ ga-adịrịrị n'ime obere akpa ahụ emechiri emechi ruo mgbe ejiri ya.

Nchịkọta na Nkwadebe ihe atụ

1. Enwere ike iji Dengue NS1 Ag-IgG/IgM Combo Test na Serum ma ọ bụ Plasma.

2. Iji nakọta ọbara zuru oke, serum ma ọ bụ plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.

3. Ekwesịrị ime nnwale ozugbo ị nwetachara ihe atụ. Ahapụla ihe nlere anya na ụlọ okpomọkụ ruo ogologo oge. Maka nchekwa ogologo oge, ekwesịrị idobe ụdị ihe n'okpuru-20 ℃.

4. Weta ụdị n'ime ụlọ okpomọkụ tupu anwale. Ihe nlere oyi kpọnwụrụ ga-agbaze kpamkpam wee gwakọta ya nke ọma tupu anwale ya. Agaghị ajụ oyi ma gbazee ihe atụ ugboro ugboro.

Usoro ule

Kwe ka ule, ụdị, ihe nchekwa na/ma ọ bụ njikwa rute ọnụ ụlọ okpomọkụ 15-30℃ (59-86℉) tupu nnwale.

1. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya. Wepu ngwaọrụ nnwale na obere akpa ahụ mechiri emechi wee jiri ya ozugbo enwere ike. Debe ngwaọrụ nnwale na elu dị ọcha na larịị.

2. Maka ule IgG/IgM: Jide dropper ahụ kwụ ọtọ wee bufee 1 dobe nke ụdị (ihe dị ka 10μl) na nke ọma (S) nke ngwaọrụ ule, wee tinye 2 tụlee nke ihe nchekwa (ihe dị ka 70μl) wee malite ngụ oge. Lee ihe atụ n'okpuru.

3. Maka Nnwale NS1: Jide dropper kwụ ọtọ ma nyefee 8 ~ 10 tụlee nke serum ma ọ bụ plasma (ihe dị ka 100μl) na ihe nlele nke ọma (S) nke ngwaọrụ ule, wee malite ngụ oge. Lee ihe atụ n'okpuru.

4. Chere ka ahịrị(s) nwere agba pụta. Gụọ nsonaazụ na nkeji iri na ise. Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.

Nkọwa nke nsonaazụ

Nke dị mma:

Maka Nnwale IgG/IgM: Ahịrị njikwa na opekata mpe otu ahịrị nnwale pụtara na akpụkpọ ahụ. Ọdịdị nke ahịrị ule T2 na-egosi ọnụnọ dengue kpọmkwem IgG mgbochi. Ọdịdị nke ahịrị ule T1 na-egosi ọnụnọ dengue kpọmkwem IgM ọgwụ mgbochi. Ma ọ bụrụ na ma T1 na T2 ahịrị pụtara, ọ na-egosi na ọnụnọ nke ma dengue kpọmkwem IgG na IgM mgbochi. Na ala nke mgbochi antibody bụ, na-esighị ike n'ihi na ahịrị.

Maka Nnwale NS1: Ahịrị abụọ pụtara. Otu ahịrị kwesịrị ịpụta mgbe niile na mpaghara akara akara (C), na nke ọzọ otu ahịrị nwere agba kwesịrị ịpụta na mpaghara ahịrị ule.

adịghị mma:

Otu ahịrị agba na-egosi na mpaghara njikwa (C) .Ọ nweghị ahịrị agba pụtara na mpaghara ahịrị ule.

Na adịghị mma: Ahịrị njikwa agaghị apụta. Oke ihe atụ ezughi oke ma ọ bụ usoro usoro na-ezighi ezi bụ ihe kachasị kpatara ọdịda akara njikwa. Nyochaa usoro ahụ wee jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ. Ọ bụrụ na nsogbu ahụ dịgidere, kwụsị iji ngwa nnwale ozugbo wee kpọtụrụ onye nkesa mpaghara gị.