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Otu nzọụkwụ HCV (Ọbara dum/Serum/Plasma)

Nkọwa dị mkpirikpi:


Nkọwa ngwaahịa

Mkpado ngwaahịa

Otu nzọụkwụ HCV (Ọbara dum/Serum/Plasma)

hcv rna
anti hcv test
hcv antibody
hcv test
hepatitis c test

Nchịkọta

Usoro n'ozuzu nke ịchọpụta ọrịa na HCV bụ ịhụ ọnụnọ nke ọgwụ nje na nje site na usoro EIA na-esote nkwenye na Western Blot. Nnwale HCV nke otu nzọụkwụ bụ ule dị mfe, nke na-achọpụta ọgwụ mgbochi ọrịa n'ọbara mmadụ dum / serum / plasma. Nnwale a dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.

Ezubere iji

Otu nzọụkwụ HCV ule bụ Colloidal Gold kwalite, ngwa ngwa Immunochromatoraphic Assay maka nchọpụta qualitative nke mgbochi nje ịba ọcha n'anya C (HCV) na ọbara mmadụ / serum / Plasma. Nnwale a bụ ule nyocha na a ga-enyocharịrị ihe niile dị mma site na iji ule ọzọ dị ka Western Blot. Ezubere ule a maka iji naanị ọkachamara nlekọta ahụike. Ma ule ahụ na nsonaazụ nnwale ahụ bụ naanị ndị ọkachamara ahụike na ndị ọka iwu ka ha ga-eji, belụsọ ma ọ bụghị iwu na obodo eji. E kwesịghị iji ule ahụ na-enweghị nlekọta kwesịrị ekwesị.

Ụkpụrụ nke usoro

Nnwale ahụ na-amalite site na nlele na-etinye aka na nke ọma na mgbakwunye nke ihe ngwọta nke enyere ozugbo. HCV antigen-Colloidal Gold conjugate nke agbakwunyere na pad sample na-emeghachi omume na mgbochi HCV dị na serum ma ọ bụ plasma, na-akpụ conjugate/HCV mgbochi mgbochi. Ka a na-ahapụ ngwakọta ahụ ịkwaga n'akụkụ eriri ule ahụ, conjugate/HCV mgbochi mgbochi bụ protein na-egbochi mmadụ na-ejide ya na akpụkpọ ahụ na-eme eriri agba na mpaghara ule. Ihe nlele na-adịghị mma anaghị ewepụta ahịrị nnwale n'ihi enweghị Colloidal Gold conjugate/HCV mgbochi mgbochi. Ihe antigens eji na ule a bụ protein recombinant kwekọrọ na mpaghara HCV nke na-enwekwa ike imeri ahụ nke ukwuu. Otu eriri njikwa agba na mpaghara njikwa na-egosi na njedebe nke usoro ule n'agbanyeghị nsonaazụ ule. Ngwa njikwa a bụ nsonaazụ Colloidal Gold conjugate na-ejikọta na mgbochi HCV na-enweghị ike imegharị na akpụkpọ ahụ. Ahịrị njikwa ahụ na-egosi na njikọta ọla edo Colloidal na-arụ ọrụ. Enweghị eriri njikwa na-egosi na ule ahụ ezighi ezi.

Reagent na ihe ndị e nyere

Nwale ngwaọrụ n'otu n'otu n'otu n'otu foil nke ejiri ihe na-ehicha ihe tinye ya

• Plastic dropper.

• Ihe nlere anya ihe atụ

Fanye ngwugwu

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

Njikwa ziri ezi na adịghị mma (dị ka ihe dị iche)

Nchekwa & Nkwụsi ike

A ga-echekwarịrị ngwa ule na 2-30 ℃ n'ime obere akpa ahụ emechiri emechi yana n'okpuru ọnọdụ akọrọ.

Ịdọ aka ná ntị na ịkpachara anya

1) A ga-akwadorịrị nsonaazụ ọma niile site na usoro ọzọ.

2) Na-emeso ụdịdị niile ka a ga-asị na ha nwere ike ibute ọrịa. Yiri uwe aka na uwe nchebe mgbe ị na-eji ihe nlere anya.

3) Ngwa eji eme nnwale kwesịrị ka ejiri akpaaka tupu ebufu ya.

4) Ejila ngwa ngwa gafere ụbọchị njedebe ha.

5) Adịla mgbanwe reagents si dị iche iche nza.

NKWUKWU NA NKWUKWU

1) Na-anakọta ụdị ọbara zuru oke / serum / Plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.

2) Nchekwa: Ọbara dum enweghị ike oyi kpọnwụrụ. Ekwesịrị idowe ihe nlele na friji ma ọ bụrụ na ejighi ya otu ụbọchị nchịkọta. Ekwesịrị ka oyi kpọnwụrụ ma ọ bụrụ na ejighi ya n'ime ụbọchị 3 nke ịnakọta. Zere oyi na agbaze ihe nlere ahụ ihe karịrị ugboro 2-3 tupu iji ya. 0.1% nke sodium Azide nwere ike ịgbakwunye na ụdị dị ka ihe nchekwa na-enweghị emetụta nsonaazụ nke nyocha ahụ.

Usoro ASsay

1) Iji rọba rọba emechi emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.

2) Tinye 2 tụlee nke Sample Diluent na nlele ahụ nke ọma, ozugbo agbakwunyere ihe atụ ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).

3) Tugharia nsonaazụ ule na nkeji iri na ise. 

310

ndetu:

1) Itinye ihe nleba anya zuru oke dị mkpa maka nsonaazụ ule dị mma. Ọ bụrụ na anaghị ahụ ọpụpụ (wetting nke akpụkpọ ahụ) na windo ule mgbe otu nkeji gachara, gbakwunye otu dobe nke diluent na nlele nke ọma.

2) Nsonaazụ dị mma nwere ike ịpụta ozugbo otu nkeji maka nlele nwere oke ọgwụ mgbochi HCV.

3) Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara

Ịgụ nsonaazụ ule

1) Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ. Na-ebelata mkpokọta mgbochi mgbochi, ka eriri ule na-esiwanye ike.

2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ. Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.

3) Nsonaazụ ezighi ezi: Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule. Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma. Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.

Mara: Ọ bụ ihe kwesịrị ekwesị ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.

OKWU

1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.

2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ. Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma. Ọbara dum enweghị ike ịsacha.

3) Akwụsịla ihe nlele ahụ. Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ. 


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