Kaseti nyocha ngwa ngwa syphilis Antibody
Ngwa ngwa nyocha ngwa ngwa syphilis TP Antibody
Nchịkọta
A na-eji usoro izugbe nke ịchọpụta ọrịa na TP maka nchọpụta in vitro qualitative nchọpụta nke Syphilis (TP) Antibody na ọbara mmadụ dum, serum ma ọ bụ plasma sample.Usoro ọla edo colloidalma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Ezubere iji
Otu ule TP otu nzọụkwụ bụ Colloidal Gold emelitere,.N'ụlọ ọgwụ, a na-ejikarị ngwaahịa a maka nchọpụta inyeaka nke ọrịa Treponema pallidum.Ngwaahịa a bụ maka naanị ndị ọrụ ahụike.
Ụkpụrụ nke usoro
Ngwaahịa ahụ na-anabata ụkpụrụ Sanwichi antigen abụọ.Mgbe ihe nlele ahụ nwere ihe mgbochi Treponema pallidum, ihe mgbochi Treponema pallidum dị na nlele ahụ na-emeghachi omume na colloidal gold akpọrọ-syphilis recombinant antigen 1 na pad njikọ iji mepụta mgbagwoju anya antigen-antibody.A na-esepụta mgbagwoju anya chromato na-aga n'ihu site na ọrụ capillary wee weghara ya site na recombinant syphilis antigen 2 nke kpuchie na mpaghara detecticn (T line) nke akpụkpọ ahụ nitrocellulose, na eriri uhie pụtara.Ihe mgbagwoju anya na-aga n'ihu na-agbago chromatograph na elu, na ọkụkọ lgY colloidal gold markker na-ejide ewu mgbochi ọkụkọ lgY mgbochi na mpaghara njikwa àgwà (C line) nke akpụkpọ anụ nitrocellulose, na eriri uhie pụtara.Mgbe ọdịnaya nke nyocha dị na nlele ahụ dị ala karịa njedebe nchọpụta kacha nta, mpaghara nchọpụta (T line) anaghị emepụta agba.
Isi ihe
1.Test mpe mpe akwa, n'otu n'otu ngwugwu na aluminum foil bag (1piece / bag, 1/5/10/25/50ibe(s)/kit)
2.Ntufu ahịhịa ahịhịa (1picce / akpa, 1/5/10/25/50 ibe (s) / kit)
3.Medical akpa mkpofu (1picce / akpa, 1/5/10/25/50 ibe (s) / kit)
4. Ntuziaka ntuziaka (1 oyiri / akpa, 1 oyiri / kit)
Mara: Ihe ndị dị na ngwa nke ọnụọgụ batch dị iche iche anaghị agbanwe agbanwe.
Akụkụ Nhọrọ
Ihe mgbaze ihe atụ (1 iberibe / akpa, 1/5/10/25/50 ibe (s) / kit)
口 Ihe mgbochi owu mmanya na-aba n'anya (1 iberibe / akpa, 1/5/10/25/50 ibe (s) / kit)
口 agịga mkpokọta ọbara (1 ibe / akpa, 1/5/10/25/50 ibe (s) / ngwa)
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Njikwa dị mma na adịghị mma (dị ka ihe dị iche)
Nchekwa & Nkwụsi ike
Ekwesịrị ịchekwa nkwakọ ngwaahịa mbụ n'ebe kpọrọ nkụ na 4-30 ℃ chebe ya pụọ na ìhè, ma ghara ifriizi.
Ịdọ aka ná ntị na ịkpachara anya
Ngwa ahụ kwụsiri ike n'ime ụbọchị njedebe nke ebipụtara na labeling.The test pad kwesịrị iji n'ime l hour n'okpuru ọnọdụ nke 4-30 ℃ na iru mmiri <65% mgbe aluminom foil akpa bụ unacated.A na-atụ aro ka iji ya ozugbo n'okpuru oke okpomọkụ ma ọ bụ ọnọdụ iru mmiri dị elu.
NKWUKWU NA NKWUKWU
2. Nchekwa ihe atụ
2.1 Ọbara dum;A na-eji tubes anticoagulant maka nchịkọta ọbara, yana nkịtịenwere ike iji ọgwụ mgbochi;ma ọ bụrụ na enweghị ike iji ihe nlele ọbara zuru oke ozugbomkpokọta, enwere ike ịchekwa ha na 2-8 Celsius C maka ụbọchị 3, enweghị ike ịmegharị ihe nlele ahụ.
2.2 Serum / Plasma: Enwere ike ịchekwa ihe nlele ahụ na 2-8 ℃ maka ụbọchị 7, ọ kwesịrị ịdịechekwara na -20 ℃ maka nchekwa ogologo oge.
3.Only na-abụghị hemolyzed samples kwesịrị iji.ga-resample.
4 A ga-emegharịgharị ihe nlele nke friji na ụlọ okpomọkụ tupu ule ahụ.Nkea ga-agbaze nke ọma, kpoo ọkụ ma gwakọta ihe atụ ndị oyi kpọnwụrụ na mbụjiri.Adịla ifriizi ma gbazee ugboro ugboro
Usoro ASsay
1) N'iji dropper rọba emechi emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.
2) Tinye 2 tụlee nke Sample Diluent na nlele nke ọma, ozugbo agbakwunyere ihe nlele ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).
3) Tugharia nsonaazụ ule na nkeji iri na ise.
ndetu:
1) Itinye ihe nleba anya zuru oke dị mkpa maka nsonaazụ ule dị mma.Ọ bụrụ na anaghị ahụ ọpụpụ (wetting nke akpụkpọ ahụ) na windo ule mgbe otu nkeji gachara, tinye otu dobe nke diluent na nlele ọma.
2) Nsonaazụ dị mma nwere ike ịpụta ozugbo otu nkeji maka nlele nwere nnukwu ọgwụ mgbochi TP.
3) Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara
Ịgụ nsonaazụ ule
1)Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ.Na-ebelata mkpokọta mgbochi, na-esiwanye ike bandwit ule.
2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.
3)Nsonaazụ ezighi ezi:Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule.Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma.Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.
Mara: Ọ bụ ihe dị mma ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.
MAKA
1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.
2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ.Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma.Ọbara dum enweghị ike ịsacha.
3) Akwụsịla ihe nlele ahụ.Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ.
