COVID-19 Neutralizing Antibody Antibody Rapid Test Cassette (Colloidal gold)
COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) bụ ngwa ngwa chromatographic immunoassay maka nchọpụta qualitative nke mgbochi mgbochi COVID-19 n'ọbara mmadụ dum, serum, ma ọ bụ plasma dị ka ihe enyemaka na nyocha nke ọnụnọ nke mgbochi mgbochi. maka COVID-19.
Akwụkwọ akụkọ coronaviruses bụ nke β genus. COVID-19 bụ nnukwu ọrịa na-efe efe nke iku ume. Ndị mmadụ na-enwekarị nsogbu. Ugbu a, ndị ọrịa bu ọrịa coronavirus ọhụrụ bụ isi ihe na-ebute ọrịa; ndị butere ọrịa asymptomatic nwekwara ike bụrụ ebe a na-efe efe. Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1 ruo 14, ọkachasị ụbọchị 3 ruo 7. Ihe ngosi ndị bụ isi gụnyere ahụ ọkụ, ike ọgwụgwụ na ụkwara akọrọ. A na-ahụ mkpọchi imi, imi na-agba agba, akpịrị akpịrị, myalgia na afọ ọsịsa n'ọnọdụ ole na ole.
The COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) bụ nnwale ngwa ngwa nke na-eji ngwakọta nke S-RBD antigen mkpuchi agba maka nchọpụta nke na-ewepụ ọgwụ mgbochi na COVID-19 n'ime ọbara mmadụ dum, serum, ma ọ bụ plasma.
The COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) bụ qualitative membrane nke dabeere na immunoassay maka nchọpụta nke na-ewepụ ọgwụ mgbochi na COVID-19 n'ime ọbara dum, serum ma ọ bụ plasma. Ejiri Angiotensin I Converting Enzyme 2 (ACE2) kpuchie akpụkpọ ahụ ahụ nke ọma na mpaghara ahịrị ule nke warara. N'oge ule, ọbara dum, serum ma ọ bụ plasma na-emeghachi omume na S-RBD conjugated colloid gold. Ngwakọta ahụ na-akwaga elu na akpụkpọ ahụ chromatographically site na capillary omume iji meghachi omume na ACE2 na akpụkpọ ahụ wee mepụta ahịrị nwere agba. Ọnụnọ nke ahịrị agba a na-egosi nsonaazụ na-adịghị mma, ebe enweghị ya na-egosi nsonaazụ dị mma. Iji jee ozi dị ka njikwa usoro, eriri agba ga-agbanwe mgbe niile site na Blue gaa na Uhie na mpaghara akara akara, na-egosi na agbakwunyere olu kwesịrị ekwesị nke ihe nlere na akpụkpọ anụ ahụ emeela.
| Ngwa ule juru n'otu n'otu | Ngwaọrụ ọ bụla nwere eriri nwere conjugates nwere agba yana reactive reactive agbasasịburu na mpaghara ndị kwekọrọ. |
| pipettes enwere ike iwepu | Maka ịgbakwunye ihe nlere anya |
| Ihe nchekwa | Phosphate buffer saline na ihe nchekwa |
| Ntinye ngwugwu | Maka ntuziaka ọrụ |
Ihe Enyere
| ●Nnwale ngwaọrụ | ● Ndị na-eme ihe |
| ● Nchekwa | ● Ntinye ngwugwu |
Ihe achọrọ mana enyeghị ya
| ●Ngwakọta ihe atụ | ● Ngụ oge |
| ●Centrifuge |
1. Maka ọkachamara in vitro diagnostic were naanị.
2. Ejila mgbe ụbọchị njedebe gosipụtara na ngwugwu ahụ. Ejila ule ma ọ bụrụ na akpa foil mebiri emebi. Ejighachila ule ọzọ.
3. The mmịpụta reagent ngwọta nwere a nnu ngwọta ma ọ bụrụ na ihe ngwọta na kọntaktị akpụkpọ ma ọ bụ anya, kpochapu na copious ichekwa mmiri.
4. Zenarị mmetọ nke ihe nlere anya site n'iji igbe nchịkọta ọhụrụ maka ụdị ọ bụla enwetara.
5. Jiri nlezianya gụọ usoro ahụ dum tupu ule.
6. Aṅụla ihe ọṅụṅụ, ma ọ bụ anwụrụ ọkụ na mpaghara ebe a na-edozi ụdị na ngwa. Jikwaa ụdịdị niile dị ka a ga-asị na ha nwere ihe na-efe efe. Leba anya na akpachapụ anya siri ike megide ihe egwu microbiological n'ime usoro a niile wee soro usoro ọkọlọtọ maka mkpofu nke ọma. Yiri uwe nchebe dị ka uwe nyocha ụlọ nyocha, gloves enwere ike iwepu na nchedo anya mgbe a na-enyocha ihe nlele.
7. Ọ bụrụ na a na-enyo enyo ọrịa na a novel coronaviruses dabere na ugbu a clinical na epidemiological enyocha njirisi nke ndị ọrụ ahụike ọha na-atụ aro, ekwesịrị ịchịkọta ihe nlere anya na njikwa ọrịa kwesịrị ekwesị maka coronaviruses ọhụrụ wee zigara ya na steeti ma ọ bụ ngalaba ahụike mpaghara maka nnwale. E kwesịghị ịnwa omenala nje n'ụdị ndị a ma ọ bụrụ na BSL 3+ dị maka ịnata na ụdị omenala.
8. Adịla mgbanwe ma ọ bụ mix reagents si dị iche iche nza.
9. iru mmiri na okpomọkụ nwere ike imetụta nsonaazụ.
10. A ga-atụfu ihe eji eme nyocha dịka iwu obodo siri dị.
1. Ekwesịrị ịchekwa ngwa ahụ na 2-30 ° C ruo mgbe njedebe ga-ebipụta na obere akpa ahụ mechiri emechi.
2. Nnwale ahụ ga-anọgide na-adị n'ime akpa mkpuchi ahụ ruo mgbe ejiri ya.
3. Adịla ifriizi.
4. Ekwesịrị iji nlezianya chebe ihe ndị dị na ngwa ahụ site na mmetọ. Ejila ma ọ bụrụ na enwere ihe akaebe nke mmetọ microbial ma ọ bụ mmiri ozuzo. Mmetọ ndu nke akụrụngwa na-ekesa, arịa ma ọ bụ reagents nwere ike ibute nsonaazụ ụgha.
Tụlee ihe ọ bụla sitere na mmadụ ka ọ na-efe efe ma jikwaa ya site na iji usoro biosafety ọkọlọtọ.
Ọbara dum nke Capillary
Sachaa aka onye ọrịa wee kwe ka akpọọ ya. Ịhịa aka n'aka na-emetụghị ntụpọ ahụ. Jiri lancet na-adịghị mma tie akpụkpọ ahụ. Hichapụ ihe ịrịba ama mbụ nke ọbara. Jiri nwayọọ na-ete aka site na nkwojiaka ruo n'ọbụ aka ruo na mkpịsị aka iji mepụta mkpọda ọbara gbara okirikiri n'elu ebe mgbapu. Tinye ihe nleba anya ọbara Fingerstick na ngwaọrụ nnwale site na iji tube capillary ma ọ bụ nkwudo.
venous ọbara dum:
Na-anakọta ihe nlere ọbara n'ime tube mkpokọta elu nke lavender, blue ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ (nwere EDTA, citrate ma ọ bụ heparin, otu n'otu n'otu na Vacutainer®) site na veinpuncture.
Plasma
Na-anakọta ihe nlere ọbara n'ime tube mkpokọta elu nke lavender, blue ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ (nwere EDTA, citrate ma ọ bụ heparin, otu n'otu n'otu na Vacutainer®) site na veinpuncture. Kewaa plasma site na centrifugation. Jiri nlezianya wepụ plasma ahụ n'ime tube etinyere akara ọhụrụ.
Serum
Na-anakọta ihe nlere ọbara n'ime tube mkpokọ ọbara ọbara (na-enweghị ọgwụ mgbochi ọbara na Vacutainer®) site na veinpuncture. Kwe ka ọbara kụọ. Kewaa serum site na centrifugation. Jiri nlezianya wepụ serum ahụ n'ime tube etinyerela akara ọhụrụ.
Nwalee ụdị ngwa ngwa o kwere mee ka ị nakọtachara. Chekwaa ụdịdị na 2°C-8°C ma ọ bụrụ na anwaleghị ya ozugbo.
Na-echekwa ihe nlele na 2°C-8°C ruo ụbọchị 5. A ga-ekporịrị ụdịdị ahụ na -20 Celsius maka nchekwa ogologo oge.
Zenarị usoro mgbakasị oyiri ọtụtụ ugboro. Tupu ịnwale, weta ụdị oyi kpọnwụrụ n'ime ụlọ ahụ nwayọ wee gwakọta nwayọ. A ga-akọwapụta ihe nlere nwere obere ihe a na-ahụ anya site na centrifugation tupu nnwale. Ejila ihe nlele na-egosi nnukwu lipemia, nnukwu hemolysis ma ọ bụ turbidity iji zere ndabichi na nkọwa nsonaazụ.
Weta ihe nlere ma nwalee ihe ndị mejupụtara ya n'ime ụlọ Gwakọta ihe atụ ahụ nke ọma tupu enyocha ya ozugbo agbazechara. Debe ngwaọrụ nnwale n'elu ala dị ọcha ma dịkwa larịị.
Maka ihe nlele ọbara niile nke capillary:
Iji jiri tube capillary: Jupụta tube capillary na nyefee ihe dị ka 50µL (ma ọ bụ 2 tụlee) nke mkpịsị aka ọbara dum ihe nlere nke ọma (S) nke ngwaọrụ ule, wee tinye 1 ntụ ọka (ihe dị ka 30 µl) nke Ihe nlere anya mmiri ozugbo banye sample ọma.
Maka nlele ọbara zuru oke:
Mejuo dobe ahụ na ihe nlele ahụ Nyefee 2 tụlee (ihe dị ka 50 µL) nke ụdị n'ime sample ọma. Ijide n'aka na enweghi afụ ikuku. Mgbe ahụNyefee 1 dobe (ihe dị ka 30 µL) nke Sample Diluent ozugbo n'ime sample ọma.
Maka nlele Plasma/ Serum:
Mejuo dobe ahụ na ihe nlele ahụ Nyefee 1 dobe (ihe dị ka 25 µL) nke ụdị n'ime sample ọma. Ijide n'aka na enweghi afụ ikuku. Mgbe ahụNyefee 1 dobe (ihe dị ka 30 µL) nke Sample Diluent ozugbo n'ime sample ọma.
Hazie ngụ oge. Gụọ nsonaazụ ya na nkeji iri na ise.Agụkwala nsonaazụ ma emechaa 20 nkeji. Ka ịgbanarị mgbagwoju anya, tụfuo ngwaọrụ nnwale mgbe ị kọwachara nsonaazụ ya
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EZIOKWU:
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Naanị otu eriri agba na-apụta na mpaghara njikwa (C). Ọ nweghị eriri nwere agba pụtara na mpaghara ule (T). |
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Rịzọlt na-adịghị mma:
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Akara agba abụọ na-apụta na akpụkpọ ahụ. Otu band na-egosi na mpaghara njikwa (C) na band ọzọ na-egosi na mpaghara ule (T). |
| * IHE: Ike nke agba na mpaghara ahịrị ule ga-adịgasị iche dabere na ntinye nke mgbochi mgbochi na COVID-19 na ụdị. Ya mere, ndò ọ bụla nke agba na mpaghara akara ule kwesịrị ka a na-ewere dị njọ. | |
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Nsonaazụ ezighi ezi:
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Otu njikwa adaghị apụta. A ga-ewepụrịrị nsonaazụ sitere na ule ọ bụla nke na-emepụtabeghị igwe njikwa n'oge agụpụtara akọwapụtara. Biko lelee usoro a wee jiri ule ọhụrụ kwugharịa. Ọ bụrụ na nsogbu ahụ dịgidere, kwụsị iji ngwa ngwa ngwa wee kpọtụrụ onye na-ekesa mpaghara gị. |
1. Njikwa ime: Nnwale a nwere njirimara njikwa arụnyere arụnyere, band C. Ahịrị C na-etolite ma gbakwunyere ihe nlere na ihe nlere. Ma ọ bụghị ya, nyochaa usoro dum wee jiri ngwaọrụ ọhụrụ megharịa ule.
2. Njikwa mpụga: Ezi Laboratory Practice na-atụ aro iji njikwa mpụga, nke dị mma na nke na-adịghị mma (ọ bụrụ na achọrọ ya), iji mesie ike na-arụ ọrụ nke ọma.
