Ihe mgbochi HIV ngwa ngwa cassette (Colloidal gold)
Ngwa ngwa ule HIV
Nchịkọta
Usoro izugbe nke ịchọpụta ọrịa bu nje HIV bụ ịhụ ọnụnọ nke nje nje site na usoro EIA na-esote nkwenye na Western Blot.Otu nzọụkwụ HIV bụ ule dị mfe, nlele anya nke na-achọpụta ọgwụ mgbochi ọrịa na ọbara dum/ serum /plasma.Nnwale a dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Ezubere iji
Nnwale nje HIV otu nzọụkwụ bụ Colloidal Gold emelitere, ngwa ngwa Immunochromatoraphic Assay maka nchọpụta qualitative nke ọgwụ mgbochi ọrịa (HIV) na ọbara mmadụ / serum / Plasma.Nnwale a bụ ule nyocha na ihe niile dị mma ga-enwerịrị ike iji ule ọzọ dị ka Western Blot.Ezubere ule a maka iji naanị ọkachamara nlekọta ahụike.Ma ule ahụ na nsonaazụ nnwale ahụ bụ naanị ndị ọkachamara ahụike na ndị ọka iwu ka ha ga-eji, belụsọ ma ọ bụghị iwu na obodo eji.E kwesịghị iji ule ahụ na-enweghị nlekọta kwesịrị ekwesị.
Ụkpụrụ nke usoro
Nnwale ahụ na-amalite site na nlele na-etinye aka na nke ọma na ntinye nke ihe nrịbama nke enyere ozugbo.HIV antigen-Colloidal Gold conjugate nke agbakwunyere na pad sample na-emeghachi omume na mgbochi nje HIV dị na serum ma ọ bụ plasma, na-akpụ conjugate/HIV mgbochi mgbochi.Ka a na-ekwe ka ngwakọta ahụ kwaga n'akụkụ ebe a na-anwale ule, conjugate/HIV antibody complex na-ejide protein nke na-ejikọta ya na akpụkpọ ahụ na-emepụta eriri agba na mpaghara ule.Ihe nlele na-adịghị mma anaghị ewepụta ahịrị ule n'ihi enweghị Colloidal Gold conjugate/HIV antibody complex.Ihe antigens eji na ule a bụ protein recombinant kwekọrọ na mpaghara nje HIV na-enwe mmetụta nke ukwuu.Otu eriri njikwa agba na mpaghara njikwa na-egosi na njedebe nke usoro ule n'agbanyeghị nsonaazụ ule.Ngwa njikwa a bụ nsonaazụ nke Colloidal Gold conjugate na-ejikọta na mgbochi nje HIV na-enweghị ike imegharị na akpụkpọ ahụ.Ahịrị njikwa ahụ na-egosi na njikọta ọla edo Colloidal na-arụ ọrụ.Enweghị eriri njikwa na-egosi na ule ahụ ezighi ezi.
Reagent na ihe ndị e nyere
Nwalee ngwaọrụ n'otu n'otu n'otu n'otu foil nke ejiri ihe na-ehicha ihe tinye ya
• Plastic dropper.
• Ihe nlere anya ihe atụ
Fanye ngwugwu
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Njikwa dị mma na adịghị mma (dị ka ihe dị iche)
Nchekwa & Nkwụsi ike
A ga-echekwarịrị ngwa ule na 2-30 ℃ n'ime obere akpa mkpuchi na n'okpuru ọnọdụ akọrọ.
Ịdọ aka ná ntị na ịkpachara anya
1) A ga-akwadorịrị nsonaazụ ọma niile site na usoro ọzọ.
2) Na-emeso ụdịdị niile ka a ga-asị na ha nwere ike ibute ọrịa.Yiri uwe aka na uwe nchebe mgbe ị na-eji ihe nlere anya.
3) Ngwa eji eme nnwale kwesịrị ka akpachapụ anya tupu ebufu ya.
4) Ejila ngwa ngwa gafere ụbọchị njedebe ha.
5) Adịla mgbanwe reagents si dị iche iche nza.
NKWUKWU NA NKWUKWU
1) Na-anakọta ụdị ọbara zuru oke / serum / Plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.
2) Nchekwa: Ọbara dum enweghị ike oyi kpọnwụrụ.Ekwesịrị idowe ihe nlele na friji ma ọ bụrụ na ejighi ya otu ụbọchị nchịkọta.Ekwesịrị ka oyi kpọnwụrụ ma ọ bụrụ na ejighi ya n'ime ụbọchị 3 nke ịnakọta.Zere oyi na agbaze ihe nlere ahụ ihe karịrị ugboro 2-3 tupu iji ya.0.1% nke sodium Azide nwere ike ịgbakwunye na ụdị dị ka ihe nchekwa na-enweghị emetụta nsonaazụ nke nyocha ahụ.
Usoro ASsay
1) N'iji dropper rọba emechi emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.
2) Tinye 2 tụlee nke Sample Diluent na nlele nke ọma, ozugbo agbakwunyere ihe nlele ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).
3) Tugharia nsonaazụ ule na nkeji iri na ise.
ndetu:
1) Itinye ihe nleba anya zuru oke dị mkpa maka nsonaazụ ule dị mma.Ọ bụrụ na anaghị ahụ ọpụpụ (wetting nke akpụkpọ ahụ) na windo ule mgbe otu nkeji gachara, tinye otu dobe nke diluent na nlele ọma.
2) Nsonaazụ dị mma nwere ike ịpụta ozugbo otu nkeji maka nlele nwere oke mgbochi nje HIV.
3) Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara
Ịgụ nsonaazụ ule
1)Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ.Na-ebelata mkpokọta mgbochi, na-esiwanye ike bandwit ule.
2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.
3)Nsonaazụ ezighi ezi:Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule.Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma.Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.
Mara: Ọ bụ ihe dị mma ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.
MAKA
1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.
2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ.Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma.Ọbara dum enweghị ike ịsacha.
3) Akwụsịla ihe nlele ahụ.Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ.
