HIV HbsAg HCV combo kaseti ule ngwa ngwa
HIV HbsAg HCV Combo Ngwa ngwa nyocha Cassette (Ọbara dum/Serum/Plasma)
Nchịkọta
Usoro izugbe nke ịchọpụta ọrịa na HCV/HIV/HbsAg bụ ịhụ ọnụnọ nke ọgwụ mgbochi nje site na usoro EIA na-esote ya na nkwenye Western Blot.Nnwale otu nzọụkwụ bụ ule dị mfe, nke na-achọpụta ọgwụ mgbochi n'ime ọbara mmadụ / serum / plasma.Nnwale a dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Ezubere iji
Nnwale Otu Nzọụkwụ bụ Colloidal Gold emelitere, ngwa ngwa Immunochromatoraphic Assay maka nchọpụta qualitative nke ọgwụ mgbochi HCV/HIV/HBsAG n'ime ọbara mmadụ dum / Serum / Plasma.Nnwale a bụ ule nyocha na ihe niile dị mma ga-enwerịrị ike iji ule ọzọ dị ka Western Blot.Ezubere ule a maka iji naanị ọkachamara nlekọta ahụike.Ma ule ahụ na nsonaazụ nnwale ahụ bụ naanị ndị ọkachamara ahụike na ndị ọka iwu ka ha ga-eji, belụsọ ma ọ bụghị iwu na obodo eji.E kwesịghị iji ule ahụ na-enweghị nlekọta kwesịrị ekwesị.
Reagent na ihe ndị e nyere
Nwalee ngwaọrụ n'otu n'otu n'otu n'otu foil nke ejiri ihe na-ehicha ihe tinye ya
• Plastic dropper.
• Ihe nlere anya ihe atụ
Fanye ngwugwu
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Njikwa dị mma na nke na-adịghị mma (dị ka ihe dị iche)
Nchekwa & Nkwụsi ike
A ga-echekwarịrị ngwa ule na 2-30 ℃ n'ime obere akpa mkpuchi na n'okpuru ọnọdụ akọrọ.
Ịdọ aka ná ntị na ịkpachara anya
1) A ga-akwadorịrị nsonaazụ ọma niile site na usoro ọzọ.
2) Na-emeso ụdịdị niile ka a ga-asị na ha nwere ike ibute ọrịa.Yiri uwe aka na uwe nchebe mgbe ị na-eji ihe nlere anya.
3) Ngwa eji eme nnwale kwesịrị ka akpachapụ anya tupu ebufu ya.
4) Ejila ngwa ngwa gafere ụbọchị njedebe ha.
5) Adịla mgbanwe reagents si dị iche iche nza.
NKWUKWU NA NKWUKWU
1) Na-anakọta ụdị ọbara zuru oke / serum / Plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.
2) Nchekwa: Ọbara dum enweghị ike oyi kpọnwụrụ.Ekwesịrị idowe ihe nlele na friji ma ọ bụrụ na ejighi ya otu ụbọchị nchịkọta.Ekwesịrị ka oyi kpọnwụrụ ma ọ bụrụ na ejighi ya n'ime ụbọchị 3 nke ịnakọta.Zere oyi na agbaze ihe nlere ahụ ihe karịrị ugboro 2-3 tupu iji ya.0.1% nke sodium Azide nwere ike ịgbakwunye na ụdị dị ka ihe nchekwa na-enweghị emetụta nsonaazụ nke nyocha ahụ.
Usoro ASsay
1) N'iji dropper plastik mechiri emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.
2) Tinye 2 tụlee nke Sample Diluent na nlele nke ọma, ozugbo agbakwunyere ihe atụ ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).
3) Tugharia nsonaazụ ule na nkeji iri na ise.
ndetu:
1) Itinye ihe nleba anya zuru oke dị mkpa maka nsonaazụ ule dị mma.Ọ bụrụ na anaghị ahụ ọpụpụ (wetting nke akpụkpọ ahụ) na windo ule mgbe otu nkeji gachara, tinye otu dobe nke diluent na nlele ọma.
2) Nsonaazụ dị mma nwere ike ịpụta ozugbo otu nkeji maka nlele nwere nnukwu ọgwụ mgbochi HCV.
3) Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara
Ịgụ nsonaazụ ule
1)Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ.Na-ebelata mkpokọta mgbochi, na-esiwanye ike bandwit ule.
2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.
3)Nsonaazụ ezighi ezi:Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule.Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma.Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.
Mara: Ọ bụ ihe dị mma ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.
MAKA
1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.
2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ.Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma.Ọbara dum enweghị ike ịsacha.
3) Akwụsịla ihe nlele ahụ.Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ.
