Ngwa ngwa nyocha ngwa ngwa nke nje HIV 1/2 (W/S/P) Mmetụta dị elu
Ngwa nyocha ngwa ngwa nke nje HIV
Nchịkọta
Usoro izugbe nke ịchọpụta ọrịa bu nje HIV bụ ịhụ ọnụnọ nke nje nje site na usoro EIA na-esote nkwenye na Western Blot.Otu nzọụkwụ HIV bụ ule dị mfe, nlele anya nke na-achọpụta ọgwụ mgbochi ọrịa na ọbara dum/ serum /plasma.Nnwale a dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Reagent na ihe ndị e nyere
Nwalee ngwaọrụ n'otu n'otu n'otu n'otu foil nke ejiri ihe na-ehicha ihe tinye ya
- Nwalee Cassette 25 pcs/igbe
- Ahịhịa plastik enwere ike iwepu 25 pcs / igbe
- Buffer 1 pcs / igbe
- Ntuziaka ntuziaka 1 pcs/igbe
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Njikwa dị mma na adịghị mma (dị ka ihe dị iche)
Nchekwa & Nkwụsi ike
A ga-echekwarịrị ngwa ule na 2-30 ℃ n'ime obere akpa mkpuchi na n'okpuru ọnọdụ akọrọ.
NKWUKWU NA NKWUKWU
1) Na-anakọta ụdị ọbara zuru oke / serum / Plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.
2) Nchekwa: Ọbara dum enweghị ike oyi kpọnwụrụ.Ekwesịrị idowe ihe nlele na friji ma ọ bụrụ na ejighi ya otu ụbọchị nchịkọta.Ekwesịrị ka oyi kpọnwụrụ ma ọ bụrụ na ejighi ya n'ime ụbọchị 3 nke ịnakọta.Zere oyi na agbaze ihe nlere ahụ ihe karịrị ugboro 2-3 tupu iji ya.0.1% nke sodium Azide nwere ike ịgbakwunye na ụdị dị ka ihe nchekwa na-enweghị emetụta nsonaazụ nke nyocha ahụ.
Usoro ASsay
1) N'iji dropper rọba emechi emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.
2) Tinye 2 tụlee nke Sample Diluent na nlele nke ọma, ozugbo agbakwunyere ihe nlele ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).
3) Tugharia nsonaazụ ule na nkeji iri na ise.
Ịgụ nsonaazụ ule
1)Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ.Na-ebelata mkpokọta mgbochi, na-esiwanye ike bandwit ule.
2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.
3)Nsonaazụ ezighi ezi:Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule.Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma.Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.
Mara: Ọ bụ ihe dị mma ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.
MAKA
1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.
2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ.Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma.Ọbara dum enweghị ike ịsacha.
3) Akwụsịla ihe nlele ahụ.Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ.
