Nje Virus Immunodeficiency mmadụ (HIV 1/2) Cassette nyocha ngwa ngwa
Ngwa nyocha ngwa ngwa nke nje HIV
Nchịkọta
Usoro izugbe nke ịchọpụta ọrịa bu nje HIV bụ ịhụ ọnụnọ nke nje nje site na usoro EIA na-esote nkwenye na Western Blot.Otu nzọụkwụ HIV bụ ule dị mfe, nlele anya nke na-achọpụta ọgwụ mgbochi ọrịa na ọbara dum/ serum /plasma.Nnwale a dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Reagent na ihe ndị e nyere
Nwalee ngwaọrụ n'otu n'otu n'otu n'otu foil nke ejiri ihe na-ehicha ihe tinye ya
- Nwalee Cassette 25 pcs/igbe
- Ahịhịa plastik enwere ike iwepu 25 pcs / igbe
- Buffer 1 pcs / igbe
- Ntuziaka ntuziaka 1 pcs/igbe
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Njikwa dị mma na adịghị mma (dị ka ihe dị iche)
Nchekwa & Nkwụsi ike
A ga-echekwarịrị ngwa ule na 2-30 ℃ n'ime obere akpa mkpuchi na n'okpuru ọnọdụ akọrọ.
NKWUKWU NA NKWUKWU
1) Na-anakọta ụdị ọbara zuru oke / serum / Plasma na-eso usoro ụlọ nyocha ụlọ ọgwụ mgbe niile.
2) Nchekwa: Ọbara dum enweghị ike oyi kpọnwụrụ.Ekwesịrị idowe ihe nlele na friji ma ọ bụrụ na ejighi ya otu ụbọchị nchịkọta.Ekwesịrị ka oyi kpọnwụrụ ma ọ bụrụ na ejighi ya n'ime ụbọchị 3 nke ịnakọta.Zere oyi na agbaze ihe nlere ahụ ihe karịrị ugboro 2-3 tupu iji ya.0.1% nke sodium Azide nwere ike ịgbakwunye na ụdị dị ka ihe nchekwa na-enweghị emetụta nsonaazụ nke nyocha ahụ.
Usoro ASsay
1) N'iji dropper rọba emechi emechi maka ihe nlele, nyefee 1 dobe (10μl) nke ọbara dum / Serum / Plasma na okirikiri okirikiri nke kaadị ule.
2) Tinye 2 tụlee nke Sample Diluent na nlele nke ọma, ozugbo agbakwunyere ihe nlele ahụ, site na dropper tip diluent vial (ma ọ bụ ọdịnaya niile sitere na otu ampule ule).
3) Tugharia nsonaazụ ule na nkeji iri na ise.
Ịgụ nsonaazụ ule
1)Nke dị mma: Ma eriri ule na-acha uhie uhie na-acha uhie uhie na eriri na-acha uhie uhie na-apụta na akpụkpọ ahụ.Na-ebelata mkpokọta mgbochi, na-esiwanye ike bandwit ule.
2) Ihe ọjọọ: Naanị eriri njikwa uhie na-acha odo odo na-apụta na akpụkpọ ahụ.Enweghị eriri ule na-egosi nsonaazụ na-adịghị mma.
3)Nsonaazụ ezighi ezi:Ekwesịrị inwe eriri njikwa uhie na-acha odo odo mgbe niile na mpaghara njikwa, n'agbanyeghị nsonaazụ ule.Ọ bụrụ na ahụghị eriri njikwa, a na-ewere ule ahụ adịghị mma.Jiri ngwaọrụ nnwale ọhụrụ megharịa ule ahụ.
Mara: Ọ bụ ihe dị mma ịnwe eriri njikwa nwere obere ihe dị mma nwere ezigbo nlele, ma ọ bụrụhaala na a na-ahụ ya nke ọma.
MAKA
1) Naanị ọbara zuru oke / Serum / Plasma doro anya, dị ọhụrụ, na-asọ n'efu ka enwere ike iji mee ule a.
2) Ihe nlele ọhụrụ kacha mma mana enwere ike iji ihe nlele oyi kpọnwụrụ.Ọ bụrụ na ajụla oyi, a ga-ahapụ ya ka ọ gbazee n'ọnọdụ kwụ ọtọ wee lelee ma ọ dị mma.Ọbara dum enweghị ike ịsacha.
3) Akwụsịla ihe nlele ahụ.Fanye pipette dị n'okpuru elu nke ihe nlele ahụ iji nakọta ihe atụ.
